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Prof. Giacomo Novara β€” Urology, University of Padova

Testicular Cancer

Germ cell neoplasms β€” Seminomas and non-seminomas

Testicular cancer is the most common malignancy in men aged 20 to 50. Although relatively rare in absolute terms, it represents an oncological model of excellence: thanks to high chemo- and radiosensitivity, the probability of cure exceeds 90%, and the current therapeutic objective is to ensure the minimum effective treatment to minimise long-term side effects.

πŸ“Š Epidemiology

2,000
New cases/year in Italy (estimate)
0.1%
Of cancer deaths β€” extremely low mortality
62,000
Patients on treatment/follow-up in Italy
>90%
Probability of long-term cure

Incidence has been increasing over recent decades, both through clinical perception and epidemiological data. One-year survival exceeds 96%; 5-year survival is approximately 93%. Precisely because these patients do not die, the therapeutic approach is oriented towards reducing side effects over the course of a still very long life ahead.

⚠️ Risk factors

πŸ”½
Cryptorchidism

The testes originate embryologically in the retroperitoneum and migrate towards the scrotum during intrauterine life. Failure to complete this migration β€” cryptorchidism β€” confers an approximately 10-fold increased risk of germ cell neoplasm compared with the general population, even after orchidopexy performed within 12–24 months of life.

πŸ”¬
Subfertility / infertility

Incidental detection of testicular nodules during ultrasound work-up for infertility is an increasingly common scenario. The association between testicular dysgenesis and neoplastic risk is well recognised.

πŸ‘Ά
Other perinatal factors

Low birth weight, prematurity, advanced maternal age at delivery, retained placenta. In clinical practice these factors are rarely elicitable from young patients' history.

πŸ”¬ Histological classification

The fundamental distinction is between germ cell tumours (malignant, oncologically relevant) and non-germ cell tumours (almost always benign).

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Seminomas

Large-cell, homogeneous germ cell tumour. Peak incidence around 20 years of age. High radiosensitivity and chemosensitivity. Excellent prognosis.

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Non-seminomas

Heterogeneous group: embryonal carcinoma, yolk sac tumour, choriocarcinoma, teratoma, mixed forms. Peak incidence around 30 years of age.

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Non-germ cell tumours

Leydig cell tumours, Sertoli cell tumours. Almost always benign. Not oncologically relevant, but important for differential diagnosis.

⭐ Clinical peculiarities β€” why it is an oncological model

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Young patients

Seminomas ~20 years, non-seminomas ~30 years. The goal is to minimise side effects over a very long remaining life.

πŸ’Š
High chemo/radiosensitivity

Allows choosing the minimum effective treatment rather than maximising therapy.

πŸ§ͺ
Tumour markers

AFP and Ξ²-HCG have no diagnostic value, but are prognostic: elevated levels = more advanced disease, worse prognosis.

πŸ—ΊοΈ
Orderly metastatic spread

Predictable and constant, thanks to the retroperitoneal origin of the testis. Allows anticipation of disease sites.

πŸ§ͺ Tumour markers

Alpha-fetoprotein (AFP)

Produced in non-seminomas (yolk sac tumour, embryonal carcinoma). Not produced in pure seminomas β€” if AFP is elevated in a "seminoma", suspect a non-seminomatous component.

  • Half-life: ~5–7 days
  • Elevated in ~50–60% of non-seminomas
  • Not diagnostic, but prognostic: higher levels = worse prognosis
  • Pre-operative measurement, then every 3–4 days post-op to monitor declining kinetics
  • Failure to normalise β†’ residual metastatic disease

Ξ²-HCG (beta subunit)

Produced in 100% of choriocarcinomas, in 40–60% of embryonal carcinomas, and in a proportion of seminomas with choriocarcinoma islands.

  • Half-life: ~1 day
  • Not diagnostic, but prognostic: higher levels = worse prognosis
  • Pre-operative measurement, then on post-op days 1, 3, 5, and 7
  • Failure to normalise β†’ residual metastatic disease

A third marker, LDH (lactate dehydrogenase), is used in combination but is less sensitive and specific than the first two.

πŸ—ΊοΈ Retroperitoneal metastatic spread β€” orderly and predictable

The testes originate from the retroperitoneum adjacent to the renal crests: the primary lymph node drainage stations are therefore retroperitoneal, not inguinal (unless scrotal violation has occurred).

Right testis
Right spermatic cord β†’ paracaval precaval interaortocaval lymph nodes
all below the renal arteries

May extend to the left (paraaortic), but rarely.

Left testis
Left spermatic cord β†’ paraaortic preaortic interaortocaval lymph nodes
all below the renal arteries

Never extends to the right (towards paracaval nodes).

⚠️ Important for clinical practice: A young man with a retroperitoneal mass and elevated markers must always raise suspicion of a testicular germ cell tumour with metastases, even if the testis appears normal on palpation. Testicular ultrasound is mandatory before any biopsy of the retroperitoneal mass.

🩺 Clinical presentation and diagnosis

In most cases it is the patient himself, or his partner, who discovers a painless swelling of the testis. The swelling is of the testis (didymus), not the epididymis β€” this is the cardinal clinical finding.

Fundamental distinction on palpation: epididymal swellings are almost always infective-inflammatory processes (epididymitis). Swellings of the testicular body must raise suspicion of neoplasia.

1
Self-examination / partner examination

Scrotal mass, generally painless and monosymptomatic. Rarely is diagnosis made through metastatic symptoms.

2
Scrotal colour Doppler ultrasound

First-line investigation. The neoplastic lesion is: (a) solid β€” fluid-containing lesions are almost always benign; (b) vascularised on Doppler. MRI may supplement in selected cases.

3
AFP and Ξ²-HCG

Mandatory pre-operative measurement. Not diagnostic, but essential as a prognostic baseline and for post-operative monitoring.

4
Total body CT (staging)

Performed after histological diagnosis. For small nodules (<1 cm) β€” probable benign neoplasm β€” may be performed after frozen section. For large masses (β‰₯3 cm), probability of carcinoma is almost certain: CT may precede surgery.

5
Semen cryopreservation

Before any treatment, in all patients. Surgery and chemotherapy may impact future seminal quality. These are young men who in the majority of cases have not yet had children.

πŸ”ͺ Treatment of the primary tumour

Radical orchiectomy via the inguinal approach

The standard treatment is removal of the testis and spermatic cord. The approach must always be inguinal, never scrotal.

🚫 Scrotal violation β€” a serious error: scrotal access alters the lymphatic drainage of the testis, causing inguinal metastases that do not belong to the natural course of the disease. This complicates both treatment and follow-up. Whenever testicular neoplasm is suspected, the approach is always inguinal.

Technique: isolation of the spermatic cord at the internal inguinal ring β†’ extraction of the gonad β†’ ligation of vessels as proximally as possible β†’ orchiectomy without opening the tunica vaginalis if the neoplasm is macroscopically obvious.

Testis-sparing surgery

Indicated for small nodules β€” particularly those found incidentally during infertility work-up β€” and in patients with a solitary testis. Rationale: lesions <1 cm are benign in 75% of cases; radical orchiectomy would be overtreatment in 3 out of 4 patients.

Technique: exploratory inguinotomy β†’ intraoperative ultrasound to localise the lesion β†’ incision of the tunica albuginea over the nodule β†’ nodule excision β†’ frozen section (30–60 min) + definitive histological examination. The albuginea is closed during the waiting period.

Frozen section: benign or non-germ cell

The testis is replaced in situ. The patient goes home with the gonad preserved. Probability of parenchymal preservation: >90% for lesions <1 cm.

Frozen section: germ cell tumour

Immediate radical orchiectomy proceeds. The conservative approach was not futile: without it, the gonad would have been removed in 75% of cases inappropriately.

⚠️ Accuracy of frozen section: ~92%. Overall error rate ~8%, equally distributed between false positives (gonad removed unnecessarily) and false negatives (gonad preserved β†’ second procedure one month later for orchiectomy). The patient must be informed of both possibilities before surgery.

Testis-sparing surgery preserves a portion of testosterone-producing parenchyma. After radical orchiectomy, testosterone deficiency can affect up to 30% of patients at one year (using a cut-off of 12 nmol/L). Preservation of 1/3–1/2 of functioning testicular tissue significantly reduces this risk.

βš•οΈ Systemic treatment β€” by scenario

Treatment depends on the histological subtype (seminoma vs non-seminoma) and the stage (absence/presence of retroperitoneal lymphadenopathy on CT).

Scenario 1 β€” Seminoma Β· CT negative
Stage I seminoma (no lymphadenopathy)

Two options: active surveillance (preferred if the patient is motivated and risk factors are absent) or 1–2 cycles of carboplatin (if rete testis invasion or diameter >4 cm). Radiotherapy is not indicated due to long-term side effects in this young population.

Scenario 2 β€” Seminoma Β· CT positive
Seminoma with retroperitoneal lymphadenopathy

3–4 cycles of BEP (Bleomycin + Etoposide + Platinum). High efficacy, low toxicity in young patients. Radiotherapy is not indicated in this setting.

Scenario 3 β€” Non-seminoma Β· CT negative
Stage I non-seminoma

If no lymphovascular invasion: active surveillance. If lymphovascular invasion is present: 1 cycle of BEP. Very rare exception: pure teratoma in the testis with pure teratoma in the retroperitoneum β†’ immediate surgery (teratoma does not respond to radio/chemotherapy).

Scenario 4 β€” Non-seminoma Β· CT positive
Non-seminoma with retroperitoneal lymphadenopathy

3–4 cycles of BEP. All retroperitoneal surgery β€” when indicated β€” is performed after chemotherapy and only when markers have normalised.

The BEP regimen (Bleomycin, Etoposide, Platinum) is the reference first-line chemotherapy: highly effective in this population. The problem is that second-line chemotherapy for refractory relapses is far less effective β€” patients with viable carcinoma remaining after BEP are those who die.

πŸ” Management of residual masses after chemotherapy

Residual masses from seminoma

Threshold: 3 cm. Masses <3 cm are surveilled. Masses >3 cm require FDG-PET: if no uptake β†’ surveillance; if uptake β†’ surgery of the residual mass (technically demanding due to dense fibrotic tissue surrounding the great vessels).

Residual masses from non-seminoma

Threshold: 1 cm. Masses <1 cm are surveilled. Masses >1 cm are always surgically resected (retroperitoneal lymphadenectomy), because the histological content is unpredictable:

β…“
Fibrosis

The patient is cured. Surgery is unnecessary but unavoidable because the content cannot be predicted before surgery.

β…“
Residual teratoma

Surgery is the only possible treatment (does not respond to radio/chemotherapy). If not resected, it will grow and transform into teratocarcinoma.

β…“
Viable carcinoma

Surgery has diagnostic value only. Treatment is second-line chemotherapy β€” prognosis is severe.

πŸ“Œ Key principle: all retroperitoneal surgery for residual masses is performed only when markers have normalised. Elevated markers = still active viable carcinoma = surgery is not the treatment.

If the retroperitoneum contains teratoma, supradiaphragmatic sites (mediastinum, lungs, lateral cervical lymph nodes) will presumably also contain teratoma. The approach is systemic: resection of all visible masses, including prosthetic replacement of the aorta and inferior vena cava when necessary.

❓ Frequently asked questions

Not necessarily. Small testicular lesions discovered incidentally (for example during an ultrasound for infertility) are benign in 75% of cases if smaller than one centimetre. The first step is always a scrotal colour Doppler ultrasound performed by an experienced urologist, followed by tumour marker measurement (AFP and Ξ²-HCG). Only histological examination β€” after surgical intervention β€” provides diagnostic certainty.
It depends on the size and location of the lesion. For small nodules (generally under one centimetre), a conservative approach called testis-sparing surgery is possible in experienced centres: only the nodule is removed and, during the procedure itself, a rapid histological examination (frozen section) is performed. If the frozen section indicates a benign or non-germ cell tumour, the testis is preserved. If it indicates a germ cell tumour, radical removal of the testis proceeds immediately. In all cases, the surgical approach is always inguinal β€” never scrotal.
Before any surgery or treatment, we always recommend semen cryopreservation. This is because surgery and chemotherapy may have an impact β€” often temporary but sometimes permanent β€” on seminal quality. Having a frozen sample before treatment ensures the possibility of using assisted reproduction techniques in the future, should it become necessary. The majority of patients recover good seminal function during follow-up, but it is better to be prepared.
Not necessarily. Many patients at stage I (no lymph node involvement at diagnosis) are managed with active surveillance β€” with periodic CT scans β€” without any additional treatment. If lymph nodes are involved, chemotherapy with the BEP regimen (Bleomycin, Etoposide, Platinum) is proposed. These are effective drugs, generally well tolerated in young patients. The fact that chemotherapy is not needed in many cases is possible precisely because these tumours respond so well to therapy when required: there is no need to treat prophylactically those who do not need it.
The remaining testis is generally sufficient to produce testosterone in adequate quantities. Approximately 5–30% of patients (depending on the cut-off used) develop testosterone deficiency during long-term follow-up β€” for this reason, testosterone measurement and gonadal function assessment are part of the follow-up protocol. In the event of deficiency, testosterone replacement therapy is effective and well tolerated. Sexual life does not change significantly for the majority of patients after unilateral orchiectomy.

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